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ion xpress rna-seq barcode adapters 1-16 kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher ion xpress rna-seq barcode adapters 1-16 kit
    Ion Xpress Rna Seq Barcode Adapters 1 16 Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ion+xpress%E2%84%A2+rna-seq+barcode+1-16+kit/ion+xpress+rna+seq+barcode+1+16+kit/pm33586340-115-14-21
    Average 90 stars, based on 1 article reviews
    ion xpress rna-seq barcode adapters 1-16 kit - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    RNA Sequencing Assay:

    Article Title: Prognostic significance of p16 protein in pancreatic ductal adenocarcinoma
    Article Snippet: Samples with an RNA quality score (RIN value) of >7.0 were used for RNA-seq. rRNA was excluded from the total RNA using RiboMinus TM Eukaryote System v2. mRNA was barcoded with Ion XpressTM RNA-Seq Barcode 1-16 kit (Thermo Fisher Scientific), and the library was generated using Ion Total RNA-Seq kit v2 (Thermo Fisher Scientific).

    Article Title: Serotonergic-dependent effects of exercise and elevated stress hormone on small non-coding RNA transcriptomics and proteomics in a mouse model of affective disorders.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Generated:

    Article Title: Prognostic significance of p16 protein in pancreatic ductal adenocarcinoma
    Article Snippet: Samples with an RNA quality score (RIN value) of >7.0 were used for RNA-seq. rRNA was excluded from the total RNA using RiboMinus TM Eukaryote System v2. mRNA was barcoded with Ion XpressTM RNA-Seq Barcode 1-16 kit (Thermo Fisher Scientific), and the library was generated using Ion Total RNA-Seq kit v2 (Thermo Fisher Scientific).

    Article Title: Serotonergic-dependent effects of exercise and elevated stress hormone on small non-coding RNA transcriptomics and proteomics in a mouse model of affective disorders.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Expressing:

    Article Title: Prognostic significance of p16 protein in pancreatic ductal adenocarcinoma
    Article Snippet: Samples with an RNA quality score (RIN value) of >7.0 were used for RNA-seq. rRNA was excluded from the total RNA using RiboMinus TM Eukaryote System v2. mRNA was barcoded with Ion XpressTM RNA-Seq Barcode 1-16 kit (Thermo Fisher Scientific), and the library was generated using Ion Total RNA-Seq kit v2 (Thermo Fisher Scientific).

    Article Title: Serotonergic-dependent effects of exercise and elevated stress hormone on small non-coding RNA transcriptomics and proteomics in a mouse model of affective disorders.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.



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    Thermo Fisher ion xpress™ rna-seq barcode 1–16 kit
    Workflow outlining the exosomal small <t>RNA</t> <t>sequencing</t> experiment and data analysis. (A) Exosomes released from GT1–7 neuronal cells found in the conditioned media were isolated using 2 different centrifugation-based protocols. Protocol 1 involves the use of differential UC and Protocol 2 involves isolating exosomes using differential UC over a continuous 10–30% Optiprep gradient. Upon exosome isolation small RNA was extracted from both cells and exosomes using TRIzol-LS followed by clean up using the column-based miRNeasy kit. The quality and quantity of the small RNA were checked by Agilent 2100 Bioanalyser. Libraries were constructed and subjected to small <t>RNA</t> <t>transcriptome</t> <t>sequencing</t> using Ion Torrent Personal Genome Machine. The initial bioinformatics analysis of pre-processing, quality assessment and sequence mapping (i.e. TMAP) were performed in Ion Torrent Suite. The generated high quality and aligned data was subjected to an in-house developed small RNA analysis pipeline that integrates BEDtools and edgeR for small RNA profiling. The pipeline automatically generates output of raw counts, normalized counts and vizualization graphics including, but not limited to, hierarchical clustering heatmap and multidimensional scaling. (B) Flowchart describing the steps of the 2 protocols used to isolate exosomes from the conditioned medium; (i) differential UC and (ii) Optiprep velocity gradient ultracentrifugation.
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    Workflow outlining the exosomal small <t>RNA</t> <t>sequencing</t> experiment and data analysis. (A) Exosomes released from GT1–7 neuronal cells found in the conditioned media were isolated using 2 different centrifugation-based protocols. Protocol 1 involves the use of differential UC and Protocol 2 involves isolating exosomes using differential UC over a continuous 10–30% Optiprep gradient. Upon exosome isolation small RNA was extracted from both cells and exosomes using TRIzol-LS followed by clean up using the column-based miRNeasy kit. The quality and quantity of the small RNA were checked by Agilent 2100 Bioanalyser. Libraries were constructed and subjected to small <t>RNA</t> <t>transcriptome</t> <t>sequencing</t> using Ion Torrent Personal Genome Machine. The initial bioinformatics analysis of pre-processing, quality assessment and sequence mapping (i.e. TMAP) were performed in Ion Torrent Suite. The generated high quality and aligned data was subjected to an in-house developed small RNA analysis pipeline that integrates BEDtools and edgeR for small RNA profiling. The pipeline automatically generates output of raw counts, normalized counts and vizualization graphics including, but not limited to, hierarchical clustering heatmap and multidimensional scaling. (B) Flowchart describing the steps of the 2 protocols used to isolate exosomes from the conditioned medium; (i) differential UC and (ii) Optiprep velocity gradient ultracentrifugation.
    Ion Xpress Rna Seq Barcode 1–16 Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Workflow outlining the exosomal small RNA sequencing experiment and data analysis. (A) Exosomes released from GT1–7 neuronal cells found in the conditioned media were isolated using 2 different centrifugation-based protocols. Protocol 1 involves the use of differential UC and Protocol 2 involves isolating exosomes using differential UC over a continuous 10–30% Optiprep gradient. Upon exosome isolation small RNA was extracted from both cells and exosomes using TRIzol-LS followed by clean up using the column-based miRNeasy kit. The quality and quantity of the small RNA were checked by Agilent 2100 Bioanalyser. Libraries were constructed and subjected to small RNA transcriptome sequencing using Ion Torrent Personal Genome Machine. The initial bioinformatics analysis of pre-processing, quality assessment and sequence mapping (i.e. TMAP) were performed in Ion Torrent Suite. The generated high quality and aligned data was subjected to an in-house developed small RNA analysis pipeline that integrates BEDtools and edgeR for small RNA profiling. The pipeline automatically generates output of raw counts, normalized counts and vizualization graphics including, but not limited to, hierarchical clustering heatmap and multidimensional scaling. (B) Flowchart describing the steps of the 2 protocols used to isolate exosomes from the conditioned medium; (i) differential UC and (ii) Optiprep velocity gradient ultracentrifugation.

    Journal: RNA Biology

    Article Title: Defining the purity of exosomes required for diagnostic profiling of small RNA suitable for biomarker discovery

    doi: 10.1080/15476286.2016.1270005

    Figure Lengend Snippet: Workflow outlining the exosomal small RNA sequencing experiment and data analysis. (A) Exosomes released from GT1–7 neuronal cells found in the conditioned media were isolated using 2 different centrifugation-based protocols. Protocol 1 involves the use of differential UC and Protocol 2 involves isolating exosomes using differential UC over a continuous 10–30% Optiprep gradient. Upon exosome isolation small RNA was extracted from both cells and exosomes using TRIzol-LS followed by clean up using the column-based miRNeasy kit. The quality and quantity of the small RNA were checked by Agilent 2100 Bioanalyser. Libraries were constructed and subjected to small RNA transcriptome sequencing using Ion Torrent Personal Genome Machine. The initial bioinformatics analysis of pre-processing, quality assessment and sequence mapping (i.e. TMAP) were performed in Ion Torrent Suite. The generated high quality and aligned data was subjected to an in-house developed small RNA analysis pipeline that integrates BEDtools and edgeR for small RNA profiling. The pipeline automatically generates output of raw counts, normalized counts and vizualization graphics including, but not limited to, hierarchical clustering heatmap and multidimensional scaling. (B) Flowchart describing the steps of the 2 protocols used to isolate exosomes from the conditioned medium; (i) differential UC and (ii) Optiprep velocity gradient ultracentrifugation.

    Article Snippet: For each individual library, RNA was ligated to adapters containing a unique index barcode (Ion Xpress™ RNA-Seq Barcode 1–16 Kit, Life Technologies) to allow libraries to be pooled during Ion Torrent sequencing (Life Technologies).

    Techniques: RNA Sequencing Assay, Isolation, Centrifugation, Construct, Sequencing, Generated

    Read length of small RNA sequencing. Read length (nucleotide) distribution after adaptor removal. Each y-axis depicts the number of sequencing reads of all samples comprising of 5 replicates in each of the respective fraction, 3 replicates of exosomes isolated from ultracentrifugation method, and 3 replicates of GT1–7 neuronal cells. Standard deviations are shown in the bar graph.

    Journal: RNA Biology

    Article Title: Defining the purity of exosomes required for diagnostic profiling of small RNA suitable for biomarker discovery

    doi: 10.1080/15476286.2016.1270005

    Figure Lengend Snippet: Read length of small RNA sequencing. Read length (nucleotide) distribution after adaptor removal. Each y-axis depicts the number of sequencing reads of all samples comprising of 5 replicates in each of the respective fraction, 3 replicates of exosomes isolated from ultracentrifugation method, and 3 replicates of GT1–7 neuronal cells. Standard deviations are shown in the bar graph.

    Article Snippet: For each individual library, RNA was ligated to adapters containing a unique index barcode (Ion Xpress™ RNA-Seq Barcode 1–16 Kit, Life Technologies) to allow libraries to be pooled during Ion Torrent sequencing (Life Technologies).

    Techniques: RNA Sequencing Assay, Sequencing, Isolation

    Small RNA sequencing of GT1–7 neuronal cells and GT1–7-derived exosomes isolated by 2 different protocols. (A) Circos diagram depicting small RNA transcriptome data. Track A: cytogenetic bands, chromosomes are depicted qter to pter. Track B to G: Highlight of all small RNAs (miRNA, rRNA, tRNA, piRNA, snRNA and snoRNA) identified across different sample groups in respect to GT1–7 neuronal cell, exosomes isolated from differential UC (protocol 1) and Optiprep fractions (i.e. Fraction 7 to 10) using Optiprep velocity gradient ultracentrifugation (protocol 2). (B) Distribution of small RNA species in the analyzed data sets. Profiles of different biotypes in each data set are percentage proportion to the total reads mapped to small RNA species and other elements, in particular to tRNA, rRNA, snoRNA, snRNA, piwi-RNA, miRNA, protein-coding fragments and others (i.e. repeat elements). Abbreviations: ‘miRNA’ – microRNA; ‘rRNA’ – rRNA; ‘tRNA’ – tRNA; ‘piRNA’ – piwi-interacting RNA; ‘snRNA’ – small nuclear RNA; ‘snoRNA’ – small nucleolar RNA; ‘UCexo’ – exosomes from differential UC.

    Journal: RNA Biology

    Article Title: Defining the purity of exosomes required for diagnostic profiling of small RNA suitable for biomarker discovery

    doi: 10.1080/15476286.2016.1270005

    Figure Lengend Snippet: Small RNA sequencing of GT1–7 neuronal cells and GT1–7-derived exosomes isolated by 2 different protocols. (A) Circos diagram depicting small RNA transcriptome data. Track A: cytogenetic bands, chromosomes are depicted qter to pter. Track B to G: Highlight of all small RNAs (miRNA, rRNA, tRNA, piRNA, snRNA and snoRNA) identified across different sample groups in respect to GT1–7 neuronal cell, exosomes isolated from differential UC (protocol 1) and Optiprep fractions (i.e. Fraction 7 to 10) using Optiprep velocity gradient ultracentrifugation (protocol 2). (B) Distribution of small RNA species in the analyzed data sets. Profiles of different biotypes in each data set are percentage proportion to the total reads mapped to small RNA species and other elements, in particular to tRNA, rRNA, snoRNA, snRNA, piwi-RNA, miRNA, protein-coding fragments and others (i.e. repeat elements). Abbreviations: ‘miRNA’ – microRNA; ‘rRNA’ – rRNA; ‘tRNA’ – tRNA; ‘piRNA’ – piwi-interacting RNA; ‘snRNA’ – small nuclear RNA; ‘snoRNA’ – small nucleolar RNA; ‘UCexo’ – exosomes from differential UC.

    Article Snippet: For each individual library, RNA was ligated to adapters containing a unique index barcode (Ion Xpress™ RNA-Seq Barcode 1–16 Kit, Life Technologies) to allow libraries to be pooled during Ion Torrent sequencing (Life Technologies).

    Techniques: RNA Sequencing Assay, Derivative Assay, Isolation